Catalog No: OKEH01427
Size:100UG
Price: $550.00
SKU
OKEH01427
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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CFB ELISA Kit (Human) : 96 Wells (OKEH01427)

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01427
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CFB ELISA Kit (Human) (OKEH01427) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFB captured in well.
ELISA Kit Range0.625 - 40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
ComponentAmount
CFB Microplate96 Wells (12 x 8 Well strips)
CFB Lyophilized Standard2
100X Biotinylated CFB Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.297 ng/mL
SpecificityNatural and recombinant Human Complement factor B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCFB
Gene Full Namecomplement factor B
Alias SymbolsBF, FB, BFD, GBG, CFAB, CFBD, PBF2, AHUS4, FBI12, H2-Bf, ARMD14
NCBI Gene Id629
Protein NameComplement factor B
Description of TargetFactor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
Swissprot IdP00751
Protein Accession #NP_001701.2
Nucleotide Accession #NM_001710.5
Protein Size (# AA)764
Molecular Weight86 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CFB.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CFB.
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