CFB ELISA Kit (Human) : 96 Wells (OKEH01427)

Catalog#: OKEH01427

• Size: 96 Wells

• Predicted Species Reactivity: Human
• Kit Detection Method: Colorimetric, OD450 nm
• Kit Duration: ~ 3 Hours
• Kit Principle: Aviva Systems Biology CFB ELISA Kit (Human) (OKEH01427) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFB captured in well.
• Kit Range: 0.625 - 40ng/mL
• Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
• Kit Reproducibility: Mean Intra-assay CV%: < 5.4% (n = 20)
  Mean Inter-assay CV%: < 7.6% (n = 20)
• Kit Component:

  Component	Amount
  CFB Microplate	96 Wells (12 x 8 Well strips)
  CFB Lyophilized Standard	2
  100X Biotinylated CFB Detector Antibody	1 x 120 uL
  100X Avidin-HRP Conjugate	1 x 120 uL
  Sample Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  25X Wash Buffer	1 x 30 mL
  TMB Substrate	1 x 10 mL
  Stop Solution	1 x 10 mL

• Reconstitution and Storage: Store as indicated in product manual.
• Gene Symbol: CFB
• Official Gene Full Name: complement factor B
• Alias Symbols: AHUS4, BF, BFD, C3/C5 convertase, CFAB, Complement factor B, FB, FBI12, GBG, Glycine-rich beta glycoprotein, H2-Bf, PBF2, Properdin factor B
• NCBI Gene Id: 629
• Protein Name: Complement factor B
• Description of Target: Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
• Swissprot Id: P00751
• Protein Accession #: NP_001701.2
• Nucleotide Accession #: NM_001710.5
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.297 ng/mL
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express CFB.
• Datasheets/Manuals: Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
• Specificity: Natural and recombinant Human Complement factor B
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Molecular Weight: 86 kDa
• Protein Size (# AA): 764

Protocol:

Tips Information: