Size:96 Wells
$469.00
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SKU
OKEH01427
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CFB ELISA Kit (Human) : 96 Wells (OKEH01427)

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Catalog#: OKEH01427
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology CFB ELISA Kit (Human) (OKEH01427) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFB captured in well.
Kit Range 0.625 - 40ng/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Kit Reproducibility Mean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
Kit Component
ComponentAmount
CFB Microplate96 Wells (12 x 8 Well strips)
CFB Lyophilized Standard2
100X Biotinylated CFB Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity 0.297 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA Datasheet Printable COA datasheet for OKEH01427
Specificity Natural and recombinant Human Complement factor B
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol CFB
Official Gene Full Name complement factor B
Alias Symbols AHUS4, BF, BFD, C3/C5 convertase, CFAB, Complement factor B, FB, FBI12, GBG, Glycine-rich beta glycoprotein, H2-Bf, PBF2, Properdin factor B
NCBI Gene Id 629
Protein Name Complement factor B
Description of Target Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
Swissprot Id P00751
Protein Accession # NP_001701.2
Nucleotide Accession # NM_001710.5
Protein Size (# AA) 764
Molecular Weight 86 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CFB.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CFB.
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