Catalog No: OKCD06843
Size:96WELLS
Price: $600.00
SKU
OKCD06843
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Datasheets/ManualsPrintable datasheet for CETP ELISA Kit (Human) (OKCD06843)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholesteryl Ester Transfer Protein (CETP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholesteryl Ester Transfer Protein (CETP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholesteryl Ester Transfer Protein (CETP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cholesteryl Ester Transfer Protein (CETP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholesteryl Ester Transfer Protein (CETP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholesteryl Ester Transfer Protein (CETP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CETP Microplate96 Wells (12 x 8 Well strips)
CETP Lyophilized Standard2
100X CETP HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 1.31ng/mL
Gene SymbolCETP
Gene Full Namecholesteryl ester transfer protein
Alias SymbolsBPI fold containing family F;BPIFF;cholesteryl ester transfer protein;cholesteryl ester transfer protein plasma;HDLCQ10;lipid transfer protein I.
NCBI Gene Id1071
Protein NameCholesteryl ester transfer protein
Description of TargetInvolved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL (PubMed:3600759, PubMed:24293641). Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination (PubMed:17237796).
Uniprot IDP11597
Protein Accession #NP_000069.2
Nucleotide Accession #NM_000078.2
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