CES5A ELISA Kit (Mouse) (OKEH03716)

Catalog No: OKEH03716

Size:96T

Mouse CES5A ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CES5A ELISA Kit (Mouse) (OKEH03716) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Ces5a antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ces5a and incubated. The Ces5a found in the sample or standards competes with the biotinylated Ces5a for limited binding sites on the immobilized anti-human Ces5a antibody. Excess unbound biotinylated Ces5a and sample or standard Ces5a is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ces5a captured in the well and inversely proportional to the amount of Ces5a which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.9% (n = 20)
  Mean Inter-assay CV%: <= 8.5% (n = 20)
• ELISA Kit Component:
  • Anti-Ces5a Microplate: 96 Wells (12 x 8 Well strips)
  • Ces5a Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Ces5a-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.16 ng/mL
• Specificity: Natural and recombinant Mouse Carboxylesterase 5A
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Ces5a
• Gene Full Name: carboxylesterase 5A
• Alias Symbols: 1700081L16Rik;1700122C07Rik;BB081581;carboxylesterase 5A;carboxylesterase 7;carboxylesterase-like urinary excreted protein homolog;cau;cauxin;Ces;Ces7;Gm503.
• NCBI Gene Id: 67935
• Protein Name: Q6AW46
• Description of Target: Carboxylesterase 5A
• Uniprot ID: https://www.uniprot.org/uniprot/Q6AW46
• Protein Accession #: NP_001003951.1
• Nucleotide Accession #: NM_001003951.2
• Protein Size (# AA): 575
• Molecular Weight: 64 kDa