CES5A ELISA Kit (Dog) (OKEH08658)

Catalog No: OKEH08658

Size:96T

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	104-114% (n = 20)	105-114% (n = 20)	109-121% (n = 20)	107-120% (n = 20)
  EDTA plasma(n=5)	96-105% (n = 20)	88-98% (n = 20)	100-110% (n = 20)	97-107% (n = 20)
  heparin plasma(n=5)	103-113% (n = 20)	88-98% (n = 20)	84-97% (n = 20)	92-104% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology CES5A ELISA Kit (Dog) (OKEH08658) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CES5A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CES5A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CES5A captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.0% (n = 20)
  Mean Inter-assay CV%: <=7.7% (n = 20)
• ELISA Kit Component:
  • CES5A Microplate: 96 Wells (12 x 8 Well strips)
  • CES5A Lyophilized Standard: 2
  • 100X Biotinylated CES5A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs.
  Subcellular Location: Secreted
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Dog Carboxylesterase 5A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CES5A
• Gene Full Name: carboxylesterase 5A
• Alias Symbols: carboxylesterase 5A;carboxylesterase 7;carboxylesterase-like urinary excreted protein homolog;cauxin;CES7.
• NCBI Gene Id: 445456
• Protein Name: Q6AW47
• Description of Target: Carboxylesterase 5A
• Uniprot ID: https://www.uniprot.org/uniprot/Q6AW47
• Protein Accession #: NP_001003969.1
• Nucleotide Accession #: NM_001003969.1