Catalog No: OKCD00758 (Formerly GWB-KBBBT6)
Size:96 Wells
Price: $725.00
SKU
OKCD00758
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)97-104%99-105%89-97%96-103%
EDTA plasma(n=5)83-97%99-105%89-96%88-96%
heparin plasma(n=5)91-98%79-104%78-92%82-101%
ELISA Kit PrincipleAviva Systems Biology Ces1e ELISA Kit (Rat) (OKCD00758) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ces1e has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ces1e is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Ces1ecaptured in well.
ELISA Kit Range31.25-2,000 pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)90-9995
EDTA plasma(n=5)94-10498
heparin plasma(n=5)95-103101
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Ces1e were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Ces1e were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-Ces1e Microplate96 Wells (12 x 8 Well Strips)
Ces1e Lyophilized Standard2 x 2 ng
100X Biotinylated Ces1e Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 14.1 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxylesterase 1 (CES1). No significant cross-reactivity or interference between Carboxylesterase 1 (CES1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCES1E
Gene Full Namecarboxylesterase 1E
Alias SymbolsCes1, Es22
NCBI Gene Id29225
Protein NameCarboxylesterase 1E
Description of TargetInvolved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs.
Swissprot IdQ63108
Protein Accession #NP_113753
Nucleotide Accession #NM_031565.2
Protein Size (# AA)561
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CES1E.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CES1E.
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