Catalog No: OKCD01667 (Formerly GWB-KBBKK7)
Size:96 Wells
Price: $675.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-101%80-104%80-102%86-94%
EDTA Plasma (n=5)85-105%80-90%99-105%89-104%
Heparin Plasma (n=5)78-99%95-103%94-102%96-103%
ELISA Kit PrincipleAviva Systems Biology CES1 ELISA Kit (Human) (OKCD01667) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CES1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CES1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CES1 captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)87-105101
EDTA Plasma (n=5)89-9793
Heparin Plasma (n=5)89-9693
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CES1 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CES1 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-CES1 Microplate96 Wells (12 x 8 Well Strips)
CES1 Lyophilized Standard2 x 10 ng
100X Biotinylated CES1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxylesterase 1 (CES1). No significant cross-reactivity or interference between Carboxylesterase 1 (CES1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCES1
Gene Full NameCarboxylesterase 1
Alias SymbolsCEH, REH, TGH, ACAT, CE-1, CES2, HMSE, SES1, HMSE1, PCE-1, hCE-1
NCBI Gene Id1066
Protein NameLiver carboxylesterase 1
Description of TargetInvolved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
Swissprot IdP23141
Protein Accession #NP_001020365.1
Nucleotide Accession #NM_001025194.1
Protein Size (# AA)567
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CES1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CES1.
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