CES1 ELISA Kit (Human) (OKCD01667)

Catalog No: OKCD01667 (Formerly GWB-KBBKK7)

Size:96WELLS

Human CES1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-101%	80-104%	80-102%	86-94%
  EDTA Plasma (n=5)	85-105%	80-90%	99-105%	89-104%
  Heparin Plasma (n=5)	78-99%	95-103%	94-102%	96-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxylesterase 1 (CES1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxylesterase 1 (CES1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxylesterase 1 (CES1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carboxylesterase 1 (CES1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	87-105	101
  EDTA Plasma (n=5)	89-97	93
  Heparin Plasma (n=5)	89-96	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxylesterase 1 (CES1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxylesterase 1 (CES1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CES1 Microplate: 96 Wells (12 x 8 Well Strips)
  • CES1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CES1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Carboxylesterase 1 (CES1). No significant cross-reactivity or interference between Carboxylesterase 1 (CES1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CES1
• Gene Full Name: carboxylesterase 1
• Alias Symbols: ACAT;acyl coenzyme A:cholesterol acyltransferase;Acyl-coenzyme A:cholesterol acyltransferase;brain carboxylesterase hBr1;Carboxylesterase 1;carboxylesterase 1 (monocyte/macrophage serine esterase 1);carboxylesterase 2 (liver);CE-1;CEH;CES2;cholesteryl ester hydrolase;cocaine carboxylesterase;egasyn;hCE-1;HMSE;HMSE1;human monocyte/macrophage serine esterase 1;liver carboxylesterase 1;methylumbelliferyl-acetate deacetylase 1;monocyte/macrophage serine esterase;PCE-1;REH;retinyl ester hydrolase;serine esterase 1;SES1;TGH;triacylglycerol hydrolase.
• NCBI Gene Id: 1066
• Protein Name: Liver carboxylesterase 1
• Description of Target: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
• Uniprot ID: P23141
• Protein Accession #: NP_001020365.1
• Nucleotide Accession #: NM_001025194.1
• Protein Size (# AA): 567