Catalog No: OKBB01240
Size:96 wells
Price: $525.00
SKU
OKBB01240
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human CES1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CES1 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: G18-L567) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CES1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
ELISA Kit Range0.78 ng/ml - 50 ng/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)14865674243231499600124678
Standard deviation62.41249.661289.1297.44306.051801.49
CV(%)4.24.45.36.55.17.3
ELISA Kit Component
ComponentAmount
96-Well Plate Pre-coated with Anti-Human CES1 Antibody96 Wells (12 x 8 Well Strips)
Lyophilized Recombinant Human CES1 Standard2 x 10 ng
100X Biotinylated Anti-Human CES1 Antibody1 x 130 uL
100X Avidin-Biotin Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Additional Information

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11486147213781422143942.745.3%
Sample 256745344594451805535295.425.3%
Sample 32432325116245962432924591322.531.3%

*number of samples for each test n=16.

::
Standard Number87654321
Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0540.1960.2570.4310.6931.2221.8402.549
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum and plasma (heparin, EDTA)
Sensitivity<0.1 ng/ml
SpecificityNatural and recombinant human CES1
ImmunogenExpression system for standard: NS0; Immunogen sequence: G18-L567
DescriptionFor quantitative detection of human CES1 in cell culture supernatants, serum and plasma (heparin, EDTA).
Gene SymbolCES1
Gene Full Namecarboxylesterase 1
Alias SymbolsLiver carboxylesterase 1; cholesterol acyltransferase; ACAT; Brain carboxylesterase hBr1; Carboxylesterase 1; CE-1; hCE-1; Cocaine carboxylesterase; Egasyn; HMSE; Methylumbelliferyl-acetate deacetylase 1; Monocyte/macrophage serine esterase; Retinyl ester hydrolase; REH; Serine esterase 1; Triacylglycerol hydrolase; TGH; CES1; CES2; SES1
NCBI Gene Id1066
Protein NameLiver carboxylesterase 1
Description of TargetLiver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. This gene is mapped to 16q12.2. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.
Swissprot IdP23141
Protein Accession #NP_001020365.1
Nucleotide Accession #NM_001025194.1
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CES1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CES1.
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