CERK ELISA Kit (Human) (OKCD09200)

Catalog No: OKCD09200

Size:96 Wells

CERK ELISA Kit (Human) (OKCD09200) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CERK ELISA Kit (Human) (OKCD09200) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CERK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CERK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CERK captured in the well.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CERK. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CERK using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • CERK Microplate: 96 Wells (12 x 8 Well strips)
  • CERK Lyophilized Standard: 2
  • 100X CERK HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 1.14ng/mL

Target Info

• Gene Symbol: CERK
• Gene Full Name: ceramide kinase
• Alias Symbols: LK4, hCERK, dA59H18.2, dA59H18.3
• NCBI Gene Id: 64781
• Protein Name: Ceramide kinase
• Description of Target: CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation.
• Uniprot ID: Q8TCT0
• Protein Accession #: NP_073603.2
• Nucleotide Accession #: NM_022766.5