CER1 Chemi-Luminescent ELISA Kit (Human) (OKCD03367)

Catalog No: OKCD03367

Size:96WELLS

Human CER1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	98-105%	88-98%	85-102%
  EDTA Plasma (n=5)	93-101%	94-101%	90-98%	88-97%
  Heparin Plasma (n=5)	94-101%	88-104%	94-101%	79-91%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Cerberus (CER). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cerberus (CER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cerberus (CER) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-101	98
  EDTA Plasma (n=5)	79-97	85
  Heparin Plasma (n=5)	91-101	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cerberus (CER) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cerberus (CER) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CER1 Microplate: 96 Wells (12 x 8 Well strips)
  • CER1 Lyophilized Standard: 2
  • 100X CER1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.2 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CER1
• Gene Full Name: cerberus 1, DAN family BMP antagonist
• Alias Symbols: cerberus;cerberus 1, cysteine knot superfamily, homolog;cerberus-related 1;cerberus-related protein;DAN domain family member 4;DAND4.
• NCBI Gene Id: 9350
• Protein Name: Cerberus
• Description of Target: Cytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak (By similarity).
• Uniprot ID: O95813
• Protein Accession #: NP_005445
• Nucleotide Accession #: NM_005454