Catalog No: OKCD04478
Size:96 Wells
Price: $675.00
SKU
OKCD04478
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)80-94%80-94%86-95%85-97%
EDTA Plasma (n=5)85-103%79-91%91-103%78-92%
Heparin Plasma (n=5)79-96%96-104%79-92%90-105%
ELISA Kit PrincipleAviva Systems Biology CER ELISA Kit (Human) (OKCD04478) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CER has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CER is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CER captured in the well.
ELISA Kit Range31.25-2,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)86-9591
EDTA Plasma (n=5)98-105102
Heparin Plasma (n=5)80-10187
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CER. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CER using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Cer Microplate96 Wells (12 x 8 Well strips)
Cer Lyophilized Standard2
100X Cer HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity13.3 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCER
Alias SymbolsDAND4
NCBI Gene Id9350
Protein Namecerberus
Description of TargetThis gene encodes a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.
Swissprot IdO95813
Protein Accession #NP_005445.1
Nucleotide Accession #NM_005454.2
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CER.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CER.
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