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Catalog No: OKCD04478
Size:96WELLS
Price: $675.00
SKU
OKCD04478
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Datasheets/ManualsPrintable datasheet for CER ELISA Kit (Human) (OKCD04478)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)80-94%80-94%86-95%85-97%
EDTA Plasma (n=5)85-103%79-91%91-103%78-92%
Heparin Plasma (n=5)79-96%96-104%79-92%90-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cerberus (CER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cerberus (CER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cerberus (CER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cerberus (CER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.25-2000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)86-9591
EDTA Plasma (n=5)98-105102
Heparin Plasma (n=5)80-10187
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cerberus (CER) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cerberus (CER) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Cer Microplate96 Wells (12 x 8 Well strips)
Cer Lyophilized Standard2
100X Cer HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity13.3 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCER1
Gene Full Namecerberus 1, DAN family BMP antagonist
Alias Symbolscerberus;cerberus 1, cysteine knot superfamily, homolog;cerberus-related 1;cerberus-related protein;DAN domain family member 4;DAND4.
NCBI Gene Id9350
Protein NameCerberus
Description of TargetCytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak (By similarity).
Uniprot IDO95813
Protein Accession #NP_005445.1
Nucleotide Accession #NM_005454.2
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