CEP290 Antibody (OAEB00100)

Catalog No: OAEB00100 (Formerly GWB-EV0481)

Size:100UG

OAEB00100 (2.5ug/ml overnight staining of cell lines OPCT-1 (A) and MDA468 (fB) with Alexa Fluor 568 (red) and nuclear counter staining with DAPI (blue). Data kindly provided by Dr S, McArdle, and obtained by Sumanjeet Malhi, John van Geest Research Centre, Nottingham, UK

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IF
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CEP290
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 86%
• Peptide Sequence: C-RNSKHLKQQQYRAEN
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:128000.
  Western Blot: Preliminary experiments gave an approx 80kDa band in Human Kidney, Testis, and HeLa lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 290kDa according to NP_079390.3. The 80kDa band was successfully blocked by incubation with the immunizing peptide.
• Reference: * Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A.; Serotype-dependent packaging of large genes in adeno-associated viral vectors results in effective gene delivery in mice.; J. Clin. Invest. 2008 May 118 (5): 1955-64.; Pubmed 18414684

Target Info

• Gene Symbol: Cep290|CEP290
• Gene Full Name: centrosomal protein 290
• Alias Symbols: 3H11Ag;b2b1454C;b2b1454Clo;b2b1752C;b2b1752Clo;Bardet-Biedl syndrome 14 protein;Bardet-Biedl syndrome 14 protein homolog;BBS14;BC004690;cancer/testis antigen 87;centrosomal protein 290kDa;centrosomal protein of 290 kDa;CT87;CTCL tumor antigen se2-2;JBTS5;LCA10;Meckel syndrome, type 4;MKS4;monoclonal antibody 3H11 antigen;nephrocystin-6;nephrocytsin-6;Np;Nphp6;POC3;POC3 centriolar protein homolog;prostate cancer antigen T21;rd16;SLSN6;tumor antigen se2-2.
• NCBI Gene Id: 216274|80184
• Protein Name: Centrosomal protein of 290 kDa
• Uniprot ID: O15078
• Protein Accession #: NP_079390.3
• Molecular Weight: 290