Size:96 Wells
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Centromere Antibody (Human) ELISA Kit (OKCA00409)

Catalog#: OKCA00409
Domestic: within 1-2 weeks delivery International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3.5 Hours
Kit Principle Aviva Systems Biology Centromere Antibody (Human) ELISA Kit (OKCA00409) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Centromere antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanAntibody is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Centromere Antibody captured in well.
Kit Reproducibility Three different samples of known Centromere,Aca Antibody concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
Intra-Assay CV: <15%
Inter-Assay CV: <15%
Kit Component
Centromere Antibody Microplate96 Wells (12 x 8 Well strips)
Negative Control1 x1 mL
Positive Control1 x 1 mL
Anti-Human Antibody HRP-Conjugate1 x 10 mL
Sample Diluent1 x 20 mL
25X Wash Buffer1 x 20 mL
Substrate A1 x 5 mL
Substrate B1 x 5 mL
Stop Solution1 x 5 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Qualitative Reverse Capture ELISA
Alias Symbols ACA
Protein Name Centromere Antibody
Description of Target Centromere Antibody
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