CENPJ ELISA Kit (Human) (OKWB00344)

Catalog No: OKWB00344

Size:96T

Human CENPJ ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-105%	86-95%	88-100%	87-98%
  EDTA Plasma (n=5)	88-94%	90-101%	84-99%	82-91%
  Heparin Plasma (n=5)	80-100%	80-98%	90-100%	88-100%

• ELISA Kit Principle: Aviva Systems Biology CENPJ ELISA Kit (Human) (OKWB00344) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CENPJ has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CENPJ is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CENPJ captured in the well.
• ELISA Kit Range: 0.781-50ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	90-102	96
  EDTA Plasma (n=5)	88-104	98
  Heparin Plasma (n=5)	85-102	97

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • CENPJ Microplate: 96 Wells (12 x 8 Well strips)
  • CENPJ Lyophilized Standard: 2 x 50 ng
  • 100X CENPJ Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.469 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CENPJ
• Alias Symbols: LAP, CPAP, LIP1, BM032, MCPH6, SASS4, SCKL4, Sas-4, CENP-J
• NCBI Gene Id: 55835
• Protein Name: Centromere protein J
• Description of Target: Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles. Required for centriole elongation and for STIL-mediated centriole amplification. May be involved in the control of centriolar-microtubule growth by acting as a regulator of tubulin release.
• Uniprot ID: Q9HC77
• Protein Accession #: NP_060921
• Nucleotide Accession #: NM_018451