CENPH Antibody - middle region : HRP (ARP58266_P050-HRP)

Catalog No: ARP58266_P050-HRP

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CENPH
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 79%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 86%; Rat: 93%
• Peptide Sequence: Synthetic peptide located within the following region: ESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKIDLDSMENSERI
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CENPH (ARP58266_P050-HRP) antibody is Catalog # AAP58266 (Previous Catalog # AAPP32865)
• Reference: Liao,W.T., Clin. Cancer Res. 13 (2 PT 1), 508-514 (2007)

Target Info

• Gene Symbol: CENPH
• Gene Full Name: Centromere protein H
• Alias Symbols: NNF1, PMF1
• NCBI Gene Id: 64946
• Protein Name: Centromere protein H
• Description of Target: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. CENPH colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: Q9H3R5
• Protein Accession #: NP_075060
• Nucleotide Accession #: NM_022909
• Protein Size (# AA): 247
• Molecular Weight: 27kDa
• Protein Interactions: CENPH; CENPK; MTAP; NDC80; NUF2; SUGT1; UBC; VCP; CENPU; CENPN; CENPA; TRIM36; CENPP; CENPO; CENPI; ELAVL1; KIF2C; PMVK;