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CENPF Antibody (OAEB00634)

100 ug
$349.00
In Stock
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Gene Symbol:
CENPF
NCBI Gene Id:
1063
Protein Name:
Centromere protein F
Swissprot Id:
P49454
Protein Accession #:
NP_057427.3
Alias Symbols:
CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein, centromere protein F, 350/400ka (mitosin), RP11-262H5.1,
Replacement Item:
This antibody may replace item sc-11292 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
P-ELISA, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CENPF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CENPF.
Predicted Species Reactivity:
Cow, Human
Predicted Homology Based on Immunogen Sequence:
Cow: 91%; Human: 92%
Complete computational species homology data:
CENPF Antibody (OAEB00634)
Peptide Sequence:
GLESKGSENCKVQ
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-CENPF antibody - OAEB00634
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:64000.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration and the product is investigative grade.
Target Reference:
* Liao H, Winkfein RJ, Mack G, Rattner JB, Yen TJ.; CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis.; J Cell Biol. 1995 Aug;130(3):507-18.; Pubmed 7542657

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