CENPF Antibody - C-terminal region (OAGA01209)

Catalog No: OAGA01209

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Raji
5% SDS PAGE
OAGA01209 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded OVCA, using CENPF (OAGA01209) antibody at 1:250 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CENPF (OAGA01209) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2500.

Product Info

• Predicted Species Reactivity: Human, Zebrafish
• Product Format: Liquid. 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, ICC, IHC-FFPE, WB
  Positive Controls: 293T, Raji
  Cellular Localization: Cytoplasm, perinuclear region, nucleus matrix, kinetochore, spindle
  
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CENPF. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: ICC/IF: 1:100~1000
  IHC-FFPE: 1:100~1000
  WB: 1:500~3000
  

Target Info

• Gene Symbol: CENPF
• Gene Full Name: centromere protein F, 350/400kDa
• Alias Symbols: CENF, hcp-1, CILD31, STROMS, PRO1779
• NCBI Gene Id: 1063
• Description of Target: This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.
• Uniprot ID: P49454
• Molecular Weight: 368 kDa