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Catalog No: OKEH03294
Size:96T
Price: $525.00
SKU
OKEH03294
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CENPB ELISA Kit (Mouse) (OKEH03294) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cenpb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cenpb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cenpb captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.8% (n = 20)
Mean Inter-assay CV%: <= 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Cenpb Microplate96 Wells (12 x 8 Well strips)
Cenpb Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Cenpb Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Mouse Major centromere autoantigen B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCenpb
Gene Full Namecentromere protein B
Alias SymbolsCENP-B;centromere autoantigen B;Centromere protein B;major centromere autoantigen B.
NCBI Gene Id12616
Protein NameP27790
Description of TargetMajor centromere autoantigen B
Uniprot IDhttps://www.uniprot.org/uniprot/P27790
Protein Accession #NP_031708.2
Nucleotide Accession #NM_007682.3
Protein Size (# AA)599
Molecular Weight65 kDa
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