CENP-A Antibody : Biotin (OASE00147-Biotin)

Catalog No: OASE00147-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 5A7-2E11
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: WB
• Additional Information: Background Info: Detects ~18kDa. Recognizes Human CENP-A
  Scientific Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The
  Centromere Protein-A, CENP-A, is a Histone H3-like
  protein that contains a C-terminal H3-like domain, which
  is required for centromere localization of CENP-A, and an
  antigenic N-terminal domain.
  CENP-A, originally isolated from HeLa cells, is essential
  for kinetochore targeting of CENP-C. In the presence of
  DNA CENP-A forms an octa-meric complex with histones
  H4, H2A, H2B. CENP-A specifically localizes to active
  centromeres and is a component of specialized
  centromeric nucleosomes, on which kinetochores are
  assembled. CENP-A is essential for nucleosomal
  packaging of centromeric DNA at interphase and functions as
  a centromere formation marker on the chromosome.
  Certificate of Analysis: 1 ug/mL was sufficient for detection of CENPA in 20ug of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Synthetic peptide corresponding to a portion of human CENP-A.
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS, 50% glycerol
• Reference: 1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318. 2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188.
  3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223.
  4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97:
  1148-1153.

Target Info

• Gene Symbol: CENPA
• Gene Full Name: centromere protein A
• Alias Symbols: CenH3, CENP-A
• NCBI Gene Id: 1058
• Uniprot ID: P49450
• Protein Accession #: NP_001035891.1