CELA3B ELISA Kit (Human) (OKCD01814)

Catalog No: OKCD01814 (Formerly GWB-KBBSX1)

Size:96WELLS

Human CELA3B ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	88-102%	87-101%	78-99%	94-101%
  EDTA Plasma (n=5)	79-101%	85-105%	93-101%	82-101%
  Heparin Plasma (n=5)	79-93%	98-105%	78-103%	84-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3B (ELA3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 3B (ELA3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3B (ELA3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Elastase 3B (ELA3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	79-103	97
  EDTA Plasma (n=5)	80-98	84
  Heparin Plasma (n=5)	99-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CELA3B Microplate: 96 Wells (12 x 8 Well Strips)
  • CELA3B Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CELA3B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.135 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Elastase 3B (ELA3B). No significant cross-reactivity or interference between Elastase 3B (ELA3B) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CELA3B
• Gene Full Name: chymotrypsin like elastase 3B
• Alias Symbols: CBPP;cholesterol-binding pancreatic protease;chymotrypsin like elastase family member 3B;chymotrypsin-like elastase family member 3B;ELA3B;elastase 3B, pancreatic;elastase IIIB;elastase-3B;fecal elastase 1;pancreatic elastase 1;pancreatic endopeptidase E;protease E;proteinase E.
• NCBI Gene Id: 23436
• Protein Name: Chymotrypsin-like elastase family member 3B
• Description of Target: Efficient protease with alanine specificity but only little elastolytic activity.
• Uniprot ID: P08861
• Protein Accession #: NP_031378.1
• Nucleotide Accession #: NM_007352.3
• Protein Size (# AA): 270