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Catalog No: OKCD00769 (Formerly GWB-KBBDD4)
Size:96WELLS
Price: $675.00
SKU
OKCD00769
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Datasheets/ManualsPrintable datasheet for CELA3A ELISA Kit (Human) (OKCD00769)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)98-105%83-99%99-105%84-104%
EDTA plasma(n=5)94-102%79-103%81-103%86-93%
heparin plasma(n=5)85-94%81-89%90-97%84-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3A (ELA3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 3A (ELA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3A (ELA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Elastase 3A (ELA3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-9894
EDTA plasma(n=5)82-9288
heparin plasma(n=5)78-10181
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastase 3A (ELA3A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastase 3A (ELA3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CELA3A Microplate96 Wells (12 x 8 Well Strips)
CELA3A Lyophilized Standard2 x 2 ng
100X Biotinylated CELA3A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 13.4 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Elastase 3A (ELA3A). No significant cross-reactivity or interference between Elastase 3A (ELA3A) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCELA3A
Gene Full Namechymotrypsin like elastase 3A
Alias Symbolschymotrypsin like elastase family member 3A;chymotrypsin-like elastase family member 3A;ELA3;ELA3A;elastase 1;elastase 3A, pancreatic;elastase IIIA;elastase-3A;protease E.
NCBI Gene Id10136
Protein NameChymotrypsin-like elastase family member 3A
Description of TargetEfficient protease with alanine specificity but only little elastolytic activity.
Uniprot IDP09093
Protein Accession #NP_005738
Nucleotide Accession #NM_005747.4
Protein Size (# AA)270
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