Catalog No: OKWB00170
Size:96T
Price: $450.00
SKU
OKWB00170
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for CELA2B ELISA Kit (Human) (OKWB00170)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-99%92-105%89-99%91-100%
EDTA Plasma (n=5)88-99%82-99%83-96%85-99%
Heparin Plasma (n=5)88-96%83-97%81-95%84-99%
ELISA Kit PrincipleAviva Systems Biology CELA2B ELISA Kit (Human) (OKWB00170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CELA2B has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CELA2B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CELA2B captured in the well.
ELISA Kit Range0.313-20ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)85-9690
EDTA Plasma (n=5)96-103100
Heparin Plasma (n=5)85-9994
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
CELA2B Microplate96 Wells (12 x 8 Well strips)
CELA2B Lyophilized Standard2 x 20 ng
100X CELA2B Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.188 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolCELA2B
Gene Full Namechymotrypsin like elastase 2B
Alias Symbolschymotrypsin like elastase family member 2B;chymotrypsin-like elastase family member 2B;ELA2B;elastase 2B;Elastase-2B;pancreatic elastase IIB (ELA2B);RP11-265F14.2.
NCBI Gene Id51032
Protein NameChymotrypsin-like elastase family member 2B
Description of TargetActs upon elastin.
Uniprot IDP08218
Protein Accession #NP_056933
Nucleotide Accession #NM_015849
Write Your Own Review
You're reviewing:CELA2B ELISA Kit (Human) (OKWB00170)
Your Rating