Catalog No: OKWB00170
Size:96T
Price: $450.00
SKU
OKWB00170
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for CELA2B ELISA Kit (Human) (OKWB00170) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology CELA2B ELISA Kit (Human) (OKWB00170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CELA2B has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CELA2B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CELA2B captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 0.313-20ng/ml | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.188 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Type: Quantitative Sandwich ELISA |
Gene Symbol | CELA2B |
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Gene Full Name | chymotrypsin like elastase 2B |
Alias Symbols | chymotrypsin like elastase family member 2B;chymotrypsin-like elastase family member 2B;ELA2B;elastase 2B;Elastase-2B;pancreatic elastase IIB (ELA2B);RP11-265F14.2. |
NCBI Gene Id | 51032 |
Protein Name | Chymotrypsin-like elastase family member 2B |
Description of Target | Acts upon elastin. |
Uniprot ID | P08218 |
Protein Accession # | NP_056933 |
Nucleotide Accession # | NM_015849 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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