CELA2A ELISA Kit (Human) (OKEH07666)

Catalog No: OKEH07666

Size:96T

Human CELA2A ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	95-104% (n = 20)	99-108% (n = 20)	100-109% (n = 20)	91-101% (n = 20)
  EDTA plasma(n=5)	113-123% (n = 20)	108-117% (n = 20)	102-112% (n = 20)	107-117% (n = 20)
  heparin plasma(n=5)	99-108% (n = 20)	108-116% (n = 20)	97-107% (n = 20)	103-111% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology CELA2A ELISA Kit (Human) (OKEH07666) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CELA2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CELA2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CELA2A captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.3% (n = 20)
  Mean Inter-assay CV%: <=9.7% (n = 20)
• ELISA Kit Component:
  • CELA2A Microplate: 96 Wells (12 x 8 Well strips)
  • CELA2A Lyophilized Standard: 2
  • 100X Biotinylated CELA2A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Acts upon elastin.
  Subcellular Location: Secreted
  Unigene: Hs.631866
  SMR: P08217
  String: 9606.ENSP00000352639
  KEGG: hsa:63036
  MIM: 609443
  Pfam: PF00089
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 36pg/mL
• Specificity: Natural and recombinant Human Chymotrypsin-like elastase family member 2A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CELA2A
• Gene Full Name: chymotrypsin like elastase 2A
• Alias Symbols: AOMS4;chymotrypsin like elastase family member 2A;chymotrypsin-like elastase family member 2A;ELA2A;elastase 2A;Elastase-2A;pancreatic elastase 2;pancreatic elastase IIA;PE-1.
• NCBI Gene Id: 63036
• Protein Name: P08217
• Description of Target: Chymotrypsin-like elastase family member 2A
• Uniprot ID: https://www.uniprot.org/uniprot/P08217
• Protein Accession #: NP_254275.1
• Nucleotide Accession #: NM_033440.2