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Catalog No: OKCD00174 (Formerly GWB-KBBWT4)
Size:96WELLS
Price: $650.00
SKU
OKCD00174
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Datasheets/ManualsPrintable datasheet for CELA1 ELISA Kit (Mouse) (OKCD00174)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)83-101%79-98%78-94%93-101%
EDTA plasma(n=5)79-92%81-97%78-101%85-95%
heparin plasma(n=5)95-103%87-104%88-102%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pancreatic Elastase 1 (ELA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pancreatic Elastase 1 (ELA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pancreatic Elastase 1 (ELA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pancreatic Elastase 1 (ELA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)90-10398
EDTA plasma(n=5)83-9388
heparin plasma(n=5)95-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pancreatic Elastase 1 (ELA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pancreatic Elastase 1 (ELA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cela1 Microplate96 Wells (12 x 8 Well Strips)
Cela1 Lyophilized Standard2 x 1 ng
100X Biotinylated Cela1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 6.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Elastase 1, Pancreatic (ELA1). No significant cross-reactivity or interference between Elastase 1, Pancreatic (ELA1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCela1
Gene Full Namechymotrypsin-like elastase family, member 1
Alias Symbols1810009A17Rik;1810062B19Rik;chymotrypsin-like elastase family member 1;El;Ela;Ela1;Ela-1;elastase 1, pancreatic;elastase-1;PC-T;PC-TsF.
NCBI Gene Id109901
Protein NameChymotrypsin-like elastase family member 1
Description of TargetActs upon elastin.
Uniprot IDQ91X79
Protein Accession #NP_291090
Nucleotide Accession #NM_033612.2
Protein Size (# AA)266
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