CECR1 ELISA Kit (Human) (OKCD09298)

Catalog No: OKCD09298

Size:96WELLS

CECR1 ELISA Kit (Human) (OKCD09298) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CECR1 Microplate: 96 Wells (12 x 8 Well strips)
  • CECR1 Lyophilized Standard: 2
  • 100X CECR1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.118ng/mL

Target Info

• Gene Symbol: ADA2
• Gene Full Name: adenosine deaminase 2
• Alias Symbols: adenosine deaminase 2;adenosine deaminase CECR1;ADGF;cat eye syndrome chromosome region, candidate 1;cat eye syndrome critical region protein 1;CECR1;IDGFL;PAN;SNEDS;VAIHS.
• NCBI Gene Id: 51816
• Protein Name: Adenosine deaminase 2
• Description of Target: Adenosine deaminase that may contribute to the degradation of extracellular adenosine, a signaling molecule that controls a variety of cellular responses. Requires elevated adenosine levels for optimal enzyme activity. Binds to cell surfaces via proteoglycans and may play a role in the regulation of cell proliferation and differentiation, independently of its enzyme activity.
• Uniprot ID: Q9NZK5
• Protein Accession #: NP_001269154.1
• Nucleotide Accession #: NM_001282225.1