CEBPA Antibody - middle region (ARP57937_P050)

Catalog No: ARP57937_P050

WB Suggested Anti-CEBPA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Additional Information: IHC Information: Prostate
  IHC Information: Colon
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CEBPA
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 79%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 79%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: RRERNNIAVRKSRDKAKQRNVETQQKVLELTSDNDRLRKRVEQLSRELDT
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CEBPA (ARP57937_P050) antibody is Catalog # AAP57937 (Previous Catalog # AAPP32348)
• Reference: Singh,S.M., (2008) Biochem. Biophys. Res. Commun. 369 (2), 692-694
• Publications:

  Borger, P. et al. Impaired translation of CCAAT/enhancer binding protein alpha mRNA in bronchial smooth muscle cells of asthmatic patients. J. Allergy Clin. Immunol. 123, 639-45 (2009). 19121862

  Miglino, N., Roth, M., Tamm, M. & Borger, P. House dust mite extract downregulates C/EBPa in asthmatic bronchial smooth muscle cells. Eur. Respir. J. 38, 50-8 (2011). 21109558

Target Info

• Gene Symbol: CEBPA
• Gene Full Name: CCAAT/enhancer binding protein (C/EBP), alpha
• Alias Symbols: CEBP, C/EBP-alpha
• NCBI Gene Id: 1050
• Protein Name: CCAAT/enhancer-binding protein alpha
• Description of Target: CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-2385 U34070.1 592-2976
• Uniprot ID: P49715
• Protein Accession #: NP_004355
• Nucleotide Accession #: NM_004364
• Protein Size (# AA): 358
• Molecular Weight: 37kDa
• Protein Interactions: CEBPB; ZBTB16; RARA; HDAC1; BHLHE41; UBC; CEBPE; BATF2; BATF3; YY1AP1; ATF5; BATF; FOSL1; FOS; DBP; CEBPG; CEBPA; ATF4; ATF3; UBE3A; SKP2; RASGRF1; C1QBP; CDKN1A; SRA1; CDK4; PIAS1; Fbxw7; GRIN3A; RAB34; NAA16; POLR1E; MRTO4; AK5; MACF1; KAT5; AKAP9; SMC1