Catalog No: OKCD01729 (Formerly GWB-KBBUS9)
Size:96WELLS
Price: $675.00
SKU
OKCD01729
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Datasheets/ManualsPrintable datasheet for CEACAM8 ELISA Kit (Human) (OKCD01729)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)94-105%91-101%78-88%91-98%
EDTA Plasma (n=5)78-92%97-105%92-101%82-89%
Heparin Plasma (n=5)99-105%83-97%98-105%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)86-9995
EDTA Plasma (n=5)85-10596
Heparin Plasma (n=5)97-104101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CEACAM8 Microplate96 Wells (12 x 8 Well Strips)
CEACAM8 Lyophilized Standard2 x 1 ng
100X Biotinylated CEACAM8 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 5.8 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8). No significant cross-reactivity or interference between Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCEACAM8
Gene Full NameCEA cell adhesion molecule 8
Alias Symbolscarcinoembryonic antigen CGM6;carcinoembryonic antigen gene family member 6;carcinoembryonic antigen related cell adhesion molecule 8;carcinoembryonic antigen-related cell adhesion molecule 8;CD66b;CD67;CD67 antigen;CGM6;NCA-95;non-specific cross-reacting antigen NCA-95.
NCBI Gene Id1088
Protein NameCarcinoembryonic antigen-related cell adhesion molecule 8
Description of TargetCell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:8776764, PubMed:2022629, PubMed:11590190). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:8776764, PubMed:2022629, PubMed:11590190). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764).
Uniprot IDP31997
Protein Accession #NP_001807.2
Nucleotide Accession #NM_001816.3
Protein Size (# AA)349
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