CEA Antibody (OAAD00138)

Catalog No: OAAD00138

Size:100 ug

Western blot At 0.5-1?g/ml with the appropriate system to detect CEA in cells and tissues.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: CEA-9
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IP
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for three month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Carcinoembryonic antigen(CEA) isolated from a human colon adenocarcinoma cell line.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 0.5-1ug/ml with the appropriate system to detect CEA in cells and tissues.
  Immunohistochemistry(P) At 0.5-2ug/ml to detect CEA in formalin fixed and paraffin embedded tissues.
  Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1. Barnett, T.; Goebel, S. J.; Nothdurft, M. A.; Elting, J. J. : Carcinoembryonic antigen family: characterization of cDNAs coding for NCA and CEA and suggestion of nonrandom sequence variation in their conserved loop-domains. Genomics 3: 59-66, 1988.
  2 Gold, P.; Freedman, S. O. : Demonstration of tumor-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques. J. Exp. Med. 121: 439-462, 1965

Target Info

• Gene Symbol: CEACAM5
• Alias Symbols: CEA, CD66e
• NCBI Gene Id: 1048
• Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 5
• Description of Target: Carcinoembryonic antigen is a complex immunoreactive glycoprotein with a molecular weight of 180, 000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
• Uniprot ID: P06731