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Catalog No: OKCD01915 (Formerly GWB-KBBCV7)
Size:96WELLS
Price: $675.00
SKU
OKCD01915
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Datasheets/ManualsPrintable datasheet for CDNF ELISA Kit (Human) (OKCD01915)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-95%84-99%78-101%87-102%
EDTA Plasma (n=5)78-101%83-90%78-101%80-104%
Heparin Plasma (n=5)92-101%87-95%90-98%82-90%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cerebral Dopamine Neurotrophic Factor (CDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cerebral Dopamine Neurotrophic Factor (CDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cerebral Dopamine Neurotrophic Factor (CDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cerebral Dopamine Neurotrophic Factor (CDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)89-10196
EDTA Plasma (n=5)84-10187
Heparin Plasma (n=5)79-8885
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cerebral Dopamine Neurotrophic Factor (CDNF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cerebral Dopamine Neurotrophic Factor (CDNF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CDNF Microplate96 Wells (12 x 8 Well Strips)
CDNF Lyophilized Standard2 x 1 ng
100X Biotinylated CDNF Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Sensitivity< 6.4 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cerebral Dopamine Neurotrophic Factor (CDNF). No significant cross-reactivity or interference between Cerebral Dopamine Neurotrophic Factor (CDNF) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCDNF
Gene Full Namecerebral dopamine neurotrophic factor
Alias Symbolsarginine-rich, mutated in early stage tumors-like 1;ARMETL1;ARMET-like protein 1;cerebral dopamine neurotrophic factor;conserved dopamine neurotrophic factor.
NCBI Gene Id441549
Protein NameCerebral dopamine neurotrophic factor
Description of TargetTrophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).
Uniprot IDQ49AH0
Protein Accession #NP_001025125.2
Nucleotide Accession #NM_001029954.2
Protein Size (# AA)187
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