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Catalog No: OCOA00021
Size:20UG
Price: $179.00
SKU
OCOA00021
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CDKN2A Protein Lysate (OCOA00021)

Datasheets/ManualsPrintable datasheet for CDKN2A Protein Lysate (OCOA00021)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCDKN2A HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCDKN2A
Gene Full Namecyclin dependent kinase inhibitor 2A
Alias Symbolsalternative reading frame;ARF;CDK4 inhibitor p16-INK4;CDK4I;CDKN2;cell cycle negative regulator beta;CMM2;cyclin-dependent kinase 4 inhibitor A;cyclin-dependent kinase inhibitor 2A;cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4);INK4;INK4A;MLM;MTS1;MTS-1;multiple tumor suppressor 1;P14;P14ARF;P16;P16INK4;P16INK4A;P16-INK4A;P19;P19ARF;TP16.
NCBI Gene Id1029
Protein NameK7PML8, NM_000077, NP_000068, P42771
Description of TargetCyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A isoform p16INK4a [Homo sapiens], Homo sapiens cyclin dependent kinase inhibitor 2A (CDKN2A), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P42771
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000068
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000077
Molecular Weight16.4 kDa
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