CDKN2A ELISA Kit (Rat) (OKEH04377)

Catalog No: OKEH04377

Size:96T

Rat CDKN2A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Cdkn2a ELISA Kit (Rat) (OKEH04377) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdkn2a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Cdkn2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdkn2a captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Component:
  • Anti-Cdkn2a Microplate: 96 Wells (12 x 8 Well Strips)
  • Cdkn2a Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Cdkn2a Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.09 ng/mL
• Specificity: Natural and recombinant Rat Cyclin-dependent kinase inhibitor 2A, isoform 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cdkn2a
• Gene Full Name: cyclin-dependent kinase inhibitor 2A
• Alias Symbols: alternative reading frame;Arf;CDK4I;cell cycle inhibitor;cell cycle regulator;Cyclin dependent kinase inhibitor 2A (p16, inhibits CDK4);cyclin-dependent kinase 4 inhibitor A;cyclin-dependent kinase inhibitor 2A;cyclin-dependent kinase inhibitor 2a p16Ink4a;cyclin-dependent kinase inhibitor 2a p19Arf;INK4A;MTS1;p16;p16Cdkn2a;p16-INK4;p16-INK4a;p19ARF.
• NCBI Gene Id: 25163
• Protein Name: Q9R0Z3
• Description of Target: Cyclin-dependent kinase inhibitor 2A
• Uniprot ID: https://www.uniprot.org/uniprot/Q9R0Z3
• Protein Accession #: NP_113738.1
• Nucleotide Accession #: NM_031550.1
• Protein Size (# AA): 159
• Molecular Weight: 17 KDa