Catalog No: OKEH00591
Size:96T
Price: $525.00
SKU
OKEH00591
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CDKN2A ELISA Kit (Human) : 96 Wells (OKEH00591)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CDKN2A ELISA Kit (Human) (OKEH00591) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDKN2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDKN2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDKN2A captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.4% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CDKN2A Microplate96 Wells (12 x 8 Well strips)
CDKN2A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CDKN2A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.98 ng/mL
SpecificityNatural and recombinant Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCDKN2A
Gene Full Namecyclin dependent kinase inhibitor 2A
Alias Symbolsalternative reading frame;ARF;CDK4 inhibitor p16-INK4;CDK4I;CDKN2;cell cycle negative regulator beta;CMM2;cyclin-dependent kinase 4 inhibitor A;cyclin-dependent kinase inhibitor 2A;cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4);INK4;INK4A;MLM;MTS1;MTS-1;multiple tumor suppressor 1;P14;P14ARF;P16;P16INK4;P16INK4A;P16-INK4A;P19;P19ARF;TP16.
NCBI Gene Id1029
Protein NameP42771
Description of TargetCyclin-dependent kinase inhibitor 2A
Uniprot IDhttps://www.uniprot.org/uniprot/P42771
Protein Accession #NP_000068.1
Nucleotide Accession #NM_000077.4
Protein Size (# AA)156
Molecular Weight16 kDa
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