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Datasheets/Manuals | Printable datasheet for CDKN2A Antibody (Phospho-Ser152) (OAAF00565) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Additional Information | Target Modification: Phospho Modification Sites: Human:S152 |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human p16-INK4a around the phosphorylation site of Ser152. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Specificity | p16-INK4a (Phospho-Ser152) antibody detects endogenous levels of p16-INK4a only when phosphorylated at serine 152. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 |
Reference | Serrano M., Nature 366:704-707(1993). Robertson K.D., Oncogene 18:3810-3820(1999). Kitagawa Y., J. Biol. Chem. 277:46289-46297(2002). |
Gene Symbol | CDKN2A |
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Gene Full Name | cyclin dependent kinase inhibitor 2A |
Alias Symbols | alternative reading frame;ARF;CDK4 inhibitor p16-INK4;CDK4I;CDKN2;cell cycle negative regulator beta;CMM2;cyclin-dependent kinase 4 inhibitor A;cyclin-dependent kinase inhibitor 2A;cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4);INK4;INK4A;MLM;MTS1;MTS-1;multiple tumor suppressor 1;P14;P14ARF;P16;P16INK4;P16INK4A;P16-INK4A;P19;P19ARF;TP16. |
NCBI Gene Id | 1029 |
Protein Name | Cyclin-dependent kinase inhibitor 2A |
Description of Target | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. |
Uniprot ID | P42771 |
Protein Accession # | NP_000068.1 |
Molecular Weight | 16 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CDKN2A Antibody (Phospho-Ser152) (OAAF00565)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "CDKN2A Antibody (Phospho-Ser152) (OAAF00565)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CDKN2A Antibody (Phospho-Ser152) (OAAF00565)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CDKN2A Antibody (Phospho-Ser152) (OAAF00565)"?
This target may also be called "alternative reading frame;ARF;CDK4 inhibitor p16-INK4;CDK4I;CDKN2;cell cycle negative regulator beta;CMM2;cyclin-dependent kinase 4 inhibitor A;cyclin-dependent kinase inhibitor 2A;cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4);INK4;INK4A;MLM;MTS1;MTS-1;multiple tumor suppressor 1;P14;P14ARF;P16;P16INK4;P16INK4A;P16-INK4A;P19;P19ARF;TP16." in publications.
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What is the shipping cost for "CDKN2A Antibody (Phospho-Ser152) (OAAF00565)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CDKN2A Antibody (Phospho-Ser152) (OAAF00565)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CDKN2A Antibody (Phospho-Ser152) (OAAF00565)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "16 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CDKN2A Antibody (Phospho-Ser152) (OAAF00565)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CDKN2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CDKN2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CDKN2A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CDKN2A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CDKN2A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CDKN2A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.