CDKN1B ELISA Kit (Human) (OKEI00176)

Catalog No: OKEI00176

Size:96 Wells

Human CDKN1B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CDKN1B ELISA Kit (Human) (OKEI00176) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDKN1B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDKN1B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CDKN1B
• ELISA Kit Range: 0.016 - 1 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CDKN1B. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CDKN1B using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • CDKN1B Microplate: 96 Wells (12 x 8 Well strips)
  • CDKN1B Lyophilized Standard: 2
  • 100X CDKN1B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 30X Wash Buffer: 1 x 30 mL
  • 100X Luminol Substrate: 1 x 10 mL
  • Substrate Diluent: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 9.375 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CDKN1B
• Alias Symbols: KIP1, MEN4, CDKN4, MEN1B, P27KIP1
• NCBI Gene Id: 1027
• Protein Name: Cyclin-dependent kinase inhibitor 1B
• Description of Target: Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
• Uniprot ID: P46527
• Protein Accession #: NP_004055
• Nucleotide Accession #: NM_004064