Catalog No: OKEH03734
Size:96T
Price: $625.00
SKU
OKEH03734
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CDKN1A ELISA Kit (Human) (OKEH03734) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDKN1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for CDKN1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDKN1A captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.5% (n = 20)
Mean Inter-assay CV%: <= 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
CDKN1A Microplate96 Wells (12 x 8 Well strips)
CDKN1A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CDKN1A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Human Cyclin-dependent kinase inhibitor 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCDKN1A
Gene Full Namecyclin dependent kinase inhibitor 1A
Alias SymbolsCAP20;CDK-interacting protein 1;CDK-interaction protein 1;CDKN1;CIP1;cyclin-dependent kinase inhibitor 1;cyclin-dependent kinase inhibitor 1A (p21, Cip1);DNA synthesis inhibitor;MDA-6;melanoma differentiation associated protein 6;Melanoma differentiation-associated protein 6;P21;p21CIP1;SDI1;WAF1;wild-type p53-activated fragment 1.
NCBI Gene Id1026
Protein NameP38936
Description of TargetCyclin-dependent kinase inhibitor 1
Uniprot IDhttps://www.uniprot.org/uniprot/P38936
Protein Accession #NP_000380.1
Nucleotide Accession #NM_000389.4
Protein Size (# AA)164
Molecular Weight18 kDa
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