CDKN1A Antibody (Phospho-Thr145) (OASG05486)

Catalog No: OASG05486

Size:100 uL

Western Blot using HeLa

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human p21 around the phosphorylation site of T145. Location: 80-160aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Thr145
• Specificity: Phospho-p21 (T145) Polyclonal Antibody detects endogenous levels of p21 protein only when phosphorylated at T145.
• Application Info: WB 1:500-1:2000
  IHC 1:100-1:300
  ELISA 1:10000

Target Info

• Gene Symbol: CDKN1A
• Gene Full Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Alias Symbols: P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1
• NCBI Gene Id: 1026; 12575;
• Description of Target: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
• Uniprot ID: P38936, P39689
• Molecular Weight: 21 kDa