CDKN1A Antibody (OASG05487)

Catalog No: OASG05487 (Formerly GWB-GTHOMS)

Size:100 uL

Western Blot using COLO205

Western Blot using HepG2, HeLa, cell

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB
  Tissue Specificity: Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
  Cellular Localization: Cytoplasm/Nucleus.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human p21 around the non-phosphorylation site of T145. Location: 80-160aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. / Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114; leading to proteasomal degradation. / Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1 (By similarity).
• Specificity: p21 Polyclonal Antibody detects endogenous levels of p21 protein.
• Application Info: WB: 1:500~2000
  ELISA: 1:20000

Target Info

• Gene Symbol: CDKN1A
• Gene Full Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Alias Symbols: P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1
• NCBI Gene Id: 1026; 12575;
• Description of Target: p21 is a protein encoded by the CDKN1A gene which is approximately 18,1 kDa. p21 is localised to the cytoplasm and nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, RET signalling, cyclins and cell cycle regulation. It may be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. It binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. It also functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. Mutations in the CDKN1A gene may result in pancreatic cancer and ovarian cancer.
• Uniprot ID: P38936, P39689
• Molecular Weight: 21 kDa