CDKN1A Antibody - N-terminal region (ARP30198_P050)

Catalog No: ARP30198_P050

Sample Type: Human Diseased Prostate.
Primary Antibody Dilution: 20ug/mL.
Color/Signal Descriptions: CDKN1A (DAB; brown), nuclei (hematoxylin; blue).
Gene Name: CDKN1A.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Sheep
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human CDKN1A
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 92%; Guinea Pig: 92%; Horse: 83%; Human: 100%; Mouse: 92%; Pig: 100%; Rabbit: 92%; Rat: 100%; Sheep: 100%;
• Peptide Sequence: Synthetic peptide located within the following region: KACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAW
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CDKN1A (ARP30198_P050) antibody is Catalog # AAP30198

Target Info

• Gene Symbol: CDKN1A
• Gene Full Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Alias Symbols: P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1
• NCBI Gene Id: 1026
• Protein Name: Cyclin-dependent kinase inhibitor 1
• Description of Target: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
• Uniprot ID: Q6FI05
• Protein Accession #: NP_510867
• Nucleotide Accession #: NM_078467
• Protein Size (# AA): 164
• Molecular Weight: 18kDa
• Protein Interactions: CCND1; PCNA; KRT31; TRIM54; TEX11; IKZF3; UBC; TRAF1; TCF4; SKP2; CCND3; CCND2; PHKG2; PHKA1; NCL; HSPA9; HSPA8; HSPA5; HSPA2; HSPA1A; HNRNPA2B1; CDK2; CDK5; CDK4; CDK3; CDC20; CCNE1; CCNB1; CCNA2; CAMK2D; ABR; GIGYF2; ANKRD17; UBR4; SNRNP200; FBXO21; PUF