CDK7 Antibody (OACA09350)

Catalog No: OACA09350

Immunofluorescent analysis of HeLa cells at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human breast cancer at dilution of 1:100

Immunohistochemistry of paraffin-embedded human breast cancer at dilution of 1:100

Immunofluorescent analysis of HeLa cells at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Cyclin-dependent kinase 7 protein (212-341AA)
• Purification: Protein G Purified
• Preservative: 0.03% Proclin 300
• Formulation: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%

Target Info

• Gene Symbol: CDK7
• Gene Full Name: cyclin dependent kinase 7
• Alias Symbols: 39 KDa protein kinase;CAK;CAK1;CDK-activating kinase 1;CDKN7;cell division protein kinase 7;cyclin-dependent kinase 7;cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase);HCAK;homolog of Xenopus MO15 Cdk-activating kinase;kinase subunit of CAK;MO15;p39MO15;serine/threonine kinase stk1;serine/threonine protein kinase 1;serine/threonine protein kinase MO15;Serine/threonine-protein kinase 1;STK1;TFIIH basal transcription factor complex kinase subunit.
• NCBI Gene Id: 1022
• Protein Name: Cyclin-dependent kinase 7
• Description of Target: Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.
• Uniprot ID: P50613
• Protein Accession #: NP_001310998
• Nucleotide Accession #: NM_001324069