CDK4 Recombinant Antibody [8H4] (OACA12666)

Catalog No: OACA12666

Size:100UL

Western Blot
Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate
All lanes: CDK4 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 21 kDa
Observed band size: 34 kDa

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Recombinant Monoclonal
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay,
  Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: A synthesized peptide derived from human CDK4
• Purification: Affinity Purified

Target Info

• Gene Symbol: CDK4
• Gene Full Name: cyclin dependent kinase 4
• Alias Symbols: cell division protein kinase 4;CMM3;cyclin-dependent kinase 4;PSK-J3.
• NCBI Gene Id: 1019
• Protein Name: Cyclin-dependent kinase 4
• Description of Target: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
• Uniprot ID: P11802