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CDK4 Antibody - C - terminal region (OAAB17082)

400ul
$390.00
In Stock
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Gene Symbol:
CDK4
NCBI Gene Id:
1019
Protein Name:
Cyclin-dependent kinase 4
Swissprot Id:
P11802
Protein Accession #:
NP_000066.1
Alias Symbols:
CDK4, Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3
Replacement Item:
This antibody may replace item sc-136241 from Santa Cruz Biotechnology.
Description of Target:
Purified Rabbit Polyclonal Antibody (Pab)
Molecular Weight:
33730 Da
Host:
Rabbit
Clonality:
Polyclonal
Application:
WB, IHC, FC, IF
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CDK4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CDK4.
Immunogen:
This CDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-305 amino acids from the C-terminal region of human CDK4.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Reconstitution and Storage:
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets/Manuals:
Printable datasheet for OAAB17082
Sample Type Confirmation:

CDK4 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Isotype:
Ig
Intended Use:
CDK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Application Info:
IF: 1:10~50
WB: 1:1000
IHC-P: 1:50~100
FC: 1:10~50
:::
Function: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex
:::
Cellular Location: Cytoplasm. Nucleus. Membrane. Note=Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus

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