CDK12 ELISA Kit (Bovine) (OKEH08656)

Catalog No: OKEH08656

Size:96T

Bovine CDK12 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CDK12 ELISA Kit (Bovine) (OKEH08656) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDK12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDK12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDK12 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.1% (n = 20)
  Mean Inter-assay CV%: <=7.1% (n = 20)
• ELISA Kit Component:
  • CDK12 Microplate: 96 Wells (12 x 8 Well strips)
  • CDK12 Lyophilized Standard: 2
  • 100X Biotinylated CDK12 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
  Subcellular Location: Nucleus Nucleus speckle Colocalized with nuclear speckles throughout interphase.
  Unigene: Bt.67418
  String: 9913.ENSBTAP00000002005
  Pfam: PF00069
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 34 pg/mL
• Specificity: Natural and recombinant Bovine Cyclin-dependent kinase 12
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CDK12
• Alias Symbols: Cell division protein kinase 12.
• Protein Name: E1BB50
• Description of Target: Cyclin-dependent kinase 12
• Uniprot ID: https://www.uniprot.org/uniprot/E1BB50