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Catalog No: OKCD00831 (Formerly GWB-KBBVE4)
Size:96WELLS
Price: $700.00
SKU
OKCD00831
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Datasheets/ManualsPrintable datasheet for CDH5 ELISA Kit (Rat) (OKCD00831)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)90-97%98-105%91-101%85-103%
EDTA plasma(n=5)78-104%78-93%88-99%79-101%
heparin plasma(n=5)95-103%99-105%89-104%89-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 5 (CDH5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 5 (CDH5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 5 (CDH5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cadherin 5 (CDH5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)87-9994
EDTA plasma(n=5)98-105102
heparin plasma(n=5)80-9384
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cadherin 5 (CDH5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cadherin 5 (CDH5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cdh5 Microplate96 Wells (12 x 8 Well Strips)
Cdh5 Lyophilized Standard2 x 1,000 ng
100X Biotinylated Cdh5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity< 6.4 ng/mL.
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cadherin 5 (CDH5). No significant cross-reactivity or interference between Cadherin 5 (CDH5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCDH5
Gene Full Namecadherin 5
Alias SymbolsCD144, 7B4, Cadherin 5 Type 2, VE-Cadherin, Cadherin,Vascular Endothelial
NCBI Gene Id307618
Protein NameCadherin 5
Description of TargetCadherins are calcium-dependent cell adhesion proteins.
Uniprot IDF1M7E5
Protein Accession #NP_001100877
Nucleotide Accession #NM_001107407.1
Protein Size (# AA)776
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