CDH13 ELISA Kit (Mouse) (OKCD02442)

Catalog No: OKCD02442

Size:96WELLS

Mouse CDH13 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-90%	80-91%	84-94%	88-101%
  EDTA Plasma (n=5)	96-103%	80-90%	91-98%	89-96%
  Heparin Plasma (n=5)	86-104%	85-92%	96-103%	81-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin, Heart (CDHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin, Heart (CDHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin, Heart (CDHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cadherin, Heart (CDHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.50-4000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	84-91	88
  EDTA Plasma (n=5)	79-105	81
  Heparin Plasma(n=5)	98-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cadherin, Heart (CDHH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cadherin, Heart (CDHH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CDH13 Microplate: 96 Wells (12 x 8 Well strips)
  • CDH13 Lyophilized Standard: 2
  • 100X Biotinylated CDH13 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 23.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cadherin, Heart (CDHH). No significant cross-reactivity or interference between Cadherin, Heart (CDHH) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cdh13
• Gene Full Name: cadherin 13
• Alias Symbols: 4932416G01Rik;cadherin-13;Cdht;H-cadherin;heart cadherin;Tca;Tcad;T-cadh;T-cadherin;Truncated cadherin.
• NCBI Gene Id: 12554
• Protein Name: Cadherin-13
• Description of Target: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.
• Uniprot ID: Q9WTR5
• Protein Accession #: NP_062681.2
• Nucleotide Accession #: NM_019707.5
• Protein Size (# AA): 714