Catalog No: OKCD07779
Size:96 Wells
Price: $750.00
SKU
OKCD07779
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CDH1 ELISA Kit (Human) (OKCD07779) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDH1 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CDH1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CDH1 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CDH1 Microplate96 Wells (12 x 8 Well strips)
CDH1 Lyophilized Standard2
100X CDH1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.055ng/mL
Gene SymbolCDH1
Gene Full Namecadherin 1
Alias SymbolsUVO, CDHE, ECAD, LCAM, Arc-1, BCDS1, CD324
NCBI Gene Id999
Protein NameCadherin-1
Description of TargetCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Swissprot IdP12830
Protein Accession #NP_001304113.1
Nucleotide Accession #NM_001317184.1
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express CDH1.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express CDH1.
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