Catalog No: OKEH07225
Size:96T
Price: $800.00
SKU
OKEH07225
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Bos taurus, Bovine | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology CDH1 ELISA Kit (Bovine) (OKEH07225) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDH1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=5.5% (n = 20) Mean Inter-assay CV%: <=10.2% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. | ||||||||||||||||||||||
:: | Subcellular Location: Cell junction Cell membrane Single-pass type I membrane protein Endosome Golgi apparatus Trans-Golgi network Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm (By similarity). Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane (By similarity). | ||||||||||||||||||||||
:: | Unigene: Bt.64827 String: 9913.ENSBTAP00000021272 | ||||||||||||||||||||||
:: | KEGG: bta:282637 Pfam: PF00028 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.078ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Bovine Cadherin-1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | CDH1 |
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Gene Full Name | cadherin 1 |
Alias Symbols | cadherin 1, type 1, E-cadherin (epithelial);cadherin-1;E-cadherin;epithelial cadherin. |
NCBI Gene Id | 282637 |
Protein Name | Q6R8F2 |
Description of Target | Cadherin-1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q6R8F2 |
Protein Accession # | NP_001002763.1 |
Nucleotide Accession # | NM_001002763.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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