CDH1 Chemi-Luminescent ELISA Kit (Human) (OKCD03365)

Catalog No: OKCD03365

Size:96WELLS

Human CDH1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	86-103%	86-103%	84-101%	85-101%
  EDTA Plasma (n=5)	87-95%	96-105%	93-101%	95-105%
  Heparin Plasma (n=5)	83-104%	78-93%	98-105%	91-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to E-cadherin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to E-cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the E-cadherin level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	91-99	95
  EDTA Plasma (n=5)	97-105	101
  Heparin Plasma (n=5)	80-101	81

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E-cadherin were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E-cadherin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CDH1 Microplate: 96 Wells (12 x 8 Well strips)
  • CDH1 Lyophilized Standard: 2
  • 100X CDH1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine, tissue homogenates and other biological fluids
• Sensitivity: 27.4 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CDH1
• Gene Full Name: cadherin 1
• Alias Symbols: Arc-1;BCDS1;cadherin 1, E-cadherin (epithelial);cadherin 1, type 1, E-cadherin (epithelial);cadherin-1;calcium-dependent adhesion protein, epithelial;CAM 120/80;CD324;CDHE;cell-CAM 120/80;ECAD;E-cadherin 1;epididymis secretory sperm binding protein;epithelial cadherin;LCAM;UVO;uvomorulin.
• NCBI Gene Id: 999
• Protein Name: Cadherin-1
• Description of Target: Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
• Uniprot ID: P12830
• Protein Accession #: NP_001304113
• Nucleotide Accession #: NM_001317184