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| Datasheets/Manuals | Printable datasheet for CDH1 Antibody (OAAD00383) - OAAD00383 |
|---|
| Predicted Species Reactivity | Human, Mouse, monkey |
|---|---|
| Clonality | Monoclonal |
| Clone | 7H12 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | WB, IHC, FC |
| Reconstitution and Storage | Store at 4C, for long term storage, store at -20C. |
| Immunogen | Purified recombinant fragment of human CDH1 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Application Info | Western Bloting: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test. |
| Reference | 1. Nat Genet. 2009 Dec;41(12):1330-4. 2. Zhonghua Zhong Liu Za Zhi. 2009 Jul;31(7):515-9. 3. J Biol Chem. 2010 Feb 26;285(9):6658-69. |
| Alias Symbols | UVO, CDHE, ECAD, LCAM, Arc-1, BCDS1, CD324 |
|---|---|
| NCBI Gene Id | 999 |
| Description of Target | E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.Tissue specificity: Non-neural epithelial tissues. |
| Uniprot ID | P12830 |
| Molecular Weight | 135kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CDH1 Antibody (OAAD00383)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, monkey".
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How long will it take to receive "CDH1 Antibody (OAAD00383)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "CDH1 Antibody (OAAD00383)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CDH1 Antibody (OAAD00383)"?
This target may also be called "UVO, CDHE, ECAD, LCAM, Arc-1, BCDS1, CD324" in publications.
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What is the shipping cost for "CDH1 Antibody (OAAD00383)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CDH1 Antibody (OAAD00383)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CDH1 Antibody (OAAD00383)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "135kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CDH1 Antibody (OAAD00383)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with ""?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with ""?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with ""?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with ""?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
