CDCP1 Chemi-Luminescent ELISA Kit (Human) (OKCD03364)

Catalog No: OKCD03364

Size:96WELLS

Human CDCP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to CUB Domain Containing Protein 1 (CDCP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CUB Domain Containing Protein 1 (CDCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the CUB Domain Containing Protein 1 (CDCP1) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CUB Domain Containing Protein 1 (CDCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CUB Domain Containing Protein 1 (CDCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CDCP1 Microplate: 96 Wells (12 x 8 Well strips)
  • CDCP1 Lyophilized Standard: 2
  • 100X CDCP1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.312 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CDCP1
• Gene Full Name: CUB domain containing protein 1
• Alias Symbols: CD318;CUB domain-containing protein 1;membrane glycoprotein gp140;SIMA135;subtractive immunization M plus HEp3-associated 135 kDa protein;transmembrane and associated with src kinases;TRASK.
• NCBI Gene Id: 64866
• Protein Name: CUB domain-containing protein 1
• Description of Target: May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
• Uniprot ID: Q9H5V8
• Protein Accession #: NP_073753
• Nucleotide Accession #: NM_022842