CDC42 Antibody (OAPB00856)

Catalog No: OAPB00856

Size:100UG

Western blot analysis of CDC42 in human brain tissue lysate with CDC42 antibody at (A) 0.5 and (B) 1 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Guinea Pig|Human|Mouse|Porcine|Rat
• Product Format: Liquid. CDC42 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: chicken
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: CDC42 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: CDC42 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CDC42. The immunogen is located within amino acids 40 - 90 of CDC42.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-CDC42 antibody is Catalog # OEPB03027
• Application Info: CDC42 antibody can be used for detection of CDC42 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Erickson JW and Cerione RA. Multiple roles for Cdc42 in cell regulation. Curr. Opin. Cell Biol.2001; 13:153-7.;Chen W, Lim HH, and Lim L. The CDC42 homologue from Caenorhabditis elegans. Complementation of yeast mutation. J. Biol. Chem.1993; 268:13280-5.;Hart MJ, Eva A, Evans T, et al. Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. Nature1991; 354:311-4.;Rohatgi R, Ma L, Miki H, et al. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell1999; 97:221-31.

Target Info

• Gene Symbol: CDC42
• Gene Full Name: cell division cycle 42
• Alias Symbols: CDC42Hs;cell division control protein 42 homolog;dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD));dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD));G25K;G25K GTP-binding protein;growth-regulating protein;GTP binding protein, 25kDa;small GTP binding protein CDC42;TKS.
• NCBI Gene Id: 998
• Protein Name: Cell division control protein 42 homolog|cell division control protein 42 homolog isoform 1 precursor [Homo sapiens]
• Description of Target: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.
• Uniprot ID: P60953
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001782