CDC42 Antibody - N-terminal region (AVARP01017_P050)

Catalog No: AVARP01017_P050

WB Suggested Anti-CDC42 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysateCDC42 is supported by BioGPS gene expression data to be expressed in 721_B

Rabbit Anti-CDC42 Antibody
Catalog Number: AVARP01017_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Rabbit Anti-CDC42 Antibody
Catalog Number: AVARP01017_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow Tissue
Observed Staining: Cytoplasm, Plasma membrane
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Dog, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CDC42
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Rabbit: 100%
• Peptide Sequence: Synthetic peptide located within the following region: DNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSP
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CDC42 (AVARP01017_P050) antibody is Catalog # AAP30406 (Previous Catalog # AAPP00929)
• Sample Type Confirmation:

  CDC42 is supported by BioGPS gene expression data to be expressed in 721_B

• Reference: Black,S.A. (2008) J. Biol. Chem. 283 (16), 10835-10847

Target Info

• Gene Symbol: CDC42
• Gene Full Name: Cell division cycle 42 (GTP binding protein, 25kDa)
• Alias Symbols: TKS, G25K, CDC42Hs
• NCBI Gene Id: 998
• Protein Name: Cell division control protein 42 homolog
• Description of Target: CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
• Uniprot ID: P60953
• Protein Accession #: NP_001782
• Nucleotide Accession #: NM_001791
• Protein Size (# AA): 191
• Molecular Weight: 21kDa
• Protein Interactions: PARD6B; CDC42EP1; CDC42EP2; PAK2; UBC; TRAF2; PAK1; ARHGDIA; BIRC2; TNK2; TCEB2; EPRS; LARS; RUVBL1; NEK6; WIPF1; WASL; RIOK3; SSX2IP; DIAPH3; BAIAP2; PAK3; ZNF420; IQGAP1; RAP1A; WAS; TGFBR2; TGFBR1; ARHGEF6; LRRK2; FGD3; ARHGAP1; SH3RF3; BCAR1; USP6; EL